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Methods & Technologies

A helpful hint from Derek Lundberg a Postdoc in the Weigal Lab. Derek provides an image reference to balance a Centrifuge without “blanks.”

Assumes equal volumes… 🔴 = 3-way symmetry; 🔵 = 2-way symmetry.

If # samples cleanly divisible by 2, use all 🔵. If # cleanly div. by 3, do all with 🔴.

If neither clean div by 2 or 3, do one group of 3, and fill the rest with balanced pairs of 2.

Who is BenchFly?

“BenchFly is the premier science video production platform designed by research and video experts. We provide both open access and private solutions to labs and companies allowing researchers worldwide to communicate via the universal language–video.”  Benchfly provides an impressive list of videos demonstrating various scientific methods. http://www.benchfly.com/video-protocols
Once at Benchfly it’s worth a look around, check out the blog, etc.

Protein Simple: An Automated Western Blotting system

Information provided by John Froehlich

Protein Simple Wes
Protein Simple Wes

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. Unfortunately, traditional western blotting, in general, is tedious to operate, prone to variability and is not very amenable to high throughput strategies. To overcome these shortcomings, the Center for Advanced Algal and Plant Phenotyping (CAAPP) has purchased an automated western blot system, termed Wes™ (Made by Protein Simple: http://www.proteinsimple.com). Specifically, Wes™ (see figure below) that automates the entire western blotting procedure and has several significant advantages over conventional western blotting such as:

  • All the manual steps are removed thus saving valuable time
  • Fully analyzed results are ready in just 3-5 hours
  • Automating the process removes variability, so results are more reproducible run to run, between users and over time.
  • Since Wes™ doesn’t do a blotting step, protein transfer inconsistencies are eliminated, giving you more quantitative data.
  • Western protocols can be standardized across users and multiple, global locations

The Wes™ will hopefully be up and running in Room 110 (Plant Biology Building) by mid December or early January 2015. Finally, procedures to use the Wes™ will be established and in place once the Wes™ is up and running thus allowing researchers to incorporate this new technology into their research plans.

Contact: John Froehlich (froehli5@msu.edu) if you have any questions regarding the Wes™ .

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Pages

  • The Hub
  • About TheCOM™
    • Testimonials
    • About The Founder: Bethany Huot
  • TheCOM Center for Educative Research™
    • Educative Research™
    • The BIOME Project
  • FAQ
  • The Whiteboard
  • Strategic Career Management (SCM)
    • SCM: Identify
    • SCM: Defining The Void
    • SCM: Commit
    • SCM: Community Perspectives
  • The Resources
    • Digital Identity Management
    • Networking & Science Communication (#SciComm)
    • Writing & Peer Review
    • Bioinformatics & Statistics
    • Methods & Technologies
    • Teaching & Learning (T&L)
      • T & L Communities
      • T & L Training Programs/Fellowships
      • T & L Career Path Prep
      • T & L Tools & Resources
    • Career Prep
    • Job Hunting
  • The Vault (Archive)
    • The File Cabinet
      • The Pub Club Files:
        • The News
        • The Pub Club
          • The Mission
          • The People
          • The Mug Club
            • The Coaster Club
          • The Python Group
          • The Publications
            • Favorite Pubs
            • Papers of Interest…
            • Scoop.it
        • 2017 Summer – Summaries & Docs
        • 2017 Spring – Summaries & Docs
        • 2016 Fall – Summaries & Docs
        • 2016 Summer – Summaries & Docs
        • 2016 Spring – Summaries & Docs
        • 2015 Fall – Summaries & Docs
        • 2015 Summer – Summaries & Docs
        • 2015 Spring – Summaries & Docs
        • 2014 Fall – Summaries & Docs

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