The Hub

The Community of Minds

  • The Hub
  • About TheCOM™
    • Testimonials
    • About The Founder: Bethany Huot
  • TheCOM Center for Educative Research™
    • Educative Research™
    • The BIOME Project
  • FAQ
  • The Whiteboard
  • Strategic Career Management (SCM)
    • SCM: Identify
    • SCM: Defining The Void
    • SCM: Commit
    • SCM: Community Perspectives
  • The Resources
    • Digital Identity Management
    • Networking & Science Communication (#SciComm)
    • Writing & Peer Review
    • Bioinformatics & Statistics
    • Methods & Technologies
    • Teaching & Learning (T&L)
      • T & L Communities
      • T & L Training Programs/Fellowships
      • T & L Career Path Prep
      • T & L Tools & Resources
    • Career Prep
    • Job Hunting
  • The Vault (Archive)
    • The File Cabinet
      • The Pub Club Files:
        • The News
        • The Pub Club
          • The Mission
          • The People
          • The Mug Club
            • The Coaster Club
          • The Python Group
          • The Publications
            • Favorite Pubs
            • Papers of Interest…
            • Scoop.it
        • 2017 Summer – Summaries & Docs
        • 2017 Spring – Summaries & Docs
        • 2016 Fall – Summaries & Docs
        • 2016 Summer – Summaries & Docs
        • 2016 Spring – Summaries & Docs
        • 2015 Fall – Summaries & Docs
        • 2015 Summer – Summaries & Docs
        • 2015 Spring – Summaries & Docs
        • 2014 Fall – Summaries & Docs

Methods & Technologies

A helpful hint from Derek Lundberg a Postdoc in the Weigal Lab. Derek provides an image reference to balance a Centrifuge without “blanks.”

Assumes equal volumes… 🔴 = 3-way symmetry; 🔵 = 2-way symmetry.

If # samples cleanly divisible by 2, use all 🔵. If # cleanly div. by 3, do all with 🔴.

If neither clean div by 2 or 3, do one group of 3, and fill the rest with balanced pairs of 2.

Who is BenchFly?

“BenchFly is the premier science video production platform designed by research and video experts. We provide both open access and private solutions to labs and companies allowing researchers worldwide to communicate via the universal language–video.”  Benchfly provides an impressive list of videos demonstrating various scientific methods. http://www.benchfly.com/video-protocols
Once at Benchfly it’s worth a look around, check out the blog, etc.

Protein Simple: An Automated Western Blotting system

Information provided by John Froehlich

Protein Simple Wes
Protein Simple Wes

Western blotting is an important technique used in cell and molecular biology. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. The technique uses three elements to accomplish this task: (1) separation by size, (2) transfer to a solid support, and (3) marking target protein using a proper primary and secondary antibody to visualize. Unfortunately, traditional western blotting, in general, is tedious to operate, prone to variability and is not very amenable to high throughput strategies. To overcome these shortcomings, the Center for Advanced Algal and Plant Phenotyping (CAAPP) has purchased an automated western blot system, termed Wes™ (Made by Protein Simple: http://www.proteinsimple.com). Specifically, Wes™ (see figure below) that automates the entire western blotting procedure and has several significant advantages over conventional western blotting such as:

  • All the manual steps are removed thus saving valuable time
  • Fully analyzed results are ready in just 3-5 hours
  • Automating the process removes variability, so results are more reproducible run to run, between users and over time.
  • Since Wes™ doesn’t do a blotting step, protein transfer inconsistencies are eliminated, giving you more quantitative data.
  • Western protocols can be standardized across users and multiple, global locations

The Wes™ will hopefully be up and running in Room 110 (Plant Biology Building) by mid December or early January 2015. Finally, procedures to use the Wes™ will be established and in place once the Wes™ is up and running thus allowing researchers to incorporate this new technology into their research plans.

Contact: John Froehlich (froehli5@msu.edu) if you have any questions regarding the Wes™ .

Share this:

  • Tweet
  • Email
  • Share on Tumblr

Leave a Reply Cancel reply

Your email address will not be published. Required fields are marked *

Pages

  • The Hub
  • About TheCOM™
    • Testimonials
    • About The Founder: Bethany Huot
  • TheCOM Center for Educative Research™
    • Educative Research™
    • The BIOME Project
  • FAQ
  • The Whiteboard
  • Strategic Career Management (SCM)
    • SCM: Identify
    • SCM: Defining The Void
    • SCM: Commit
    • SCM: Community Perspectives
  • The Resources
    • Digital Identity Management
    • Networking & Science Communication (#SciComm)
    • Writing & Peer Review
    • Bioinformatics & Statistics
    • Methods & Technologies
    • Teaching & Learning (T&L)
      • T & L Communities
      • T & L Training Programs/Fellowships
      • T & L Career Path Prep
      • T & L Tools & Resources
    • Career Prep
    • Job Hunting
  • The Vault (Archive)
    • The File Cabinet
      • The Pub Club Files:
        • The News
        • The Pub Club
          • The Mission
          • The People
          • The Mug Club
            • The Coaster Club
          • The Python Group
          • The Publications
            • Favorite Pubs
            • Papers of Interest…
            • Scoop.it
        • 2017 Summer – Summaries & Docs
        • 2017 Spring – Summaries & Docs
        • 2016 Fall – Summaries & Docs
        • 2016 Summer – Summaries & Docs
        • 2016 Spring – Summaries & Docs
        • 2015 Fall – Summaries & Docs
        • 2015 Summer – Summaries & Docs
        • 2015 Spring – Summaries & Docs
        • 2014 Fall – Summaries & Docs

The Hub: By Extension

Science / Careers Articles

Looking for meaning in your career? Try a pay-it-forward plan
1 March 2021
Looking for meaning in your career? Try a pay-it-forward plan

In this installment of Your Unicorn Career, our columnist encourages making a habit of helping others [...]

New, more inclusive journal policies ease author name changes on published papers
25 February 2021
New, more inclusive journal policies ease author name changes on published papers

The shift will help researchers who change their names midcareer, including transgender scientists [...]

How I transformed myself into a confident presenter—thanks to Lady Gaga
25 February 2021
How I transformed myself into a confident presenter—thanks to Lady Gaga

This Ph.D. student struggled with self-doubt until she adopted a strategy inspired by one of her favorite performers [...]

Navigating Ph.D. recruitment virtual ‘visits’
24 February 2021
Navigating Ph.D. recruitment virtual ‘visits’

Checking out prospective programs from the comfort of your computer? Our Experimental Error columnist offers his advice [...]

Mentors, don’t neglect scientific trainees. Treat them as you’d like to be treated
18 February 2021
Mentors, don’t neglect scientific trainees. Treat them as you’d like to be treated

This Ph.D. student shares three tips for being a thoughtful, supportive mentor [...]

Benchfly / Career Development

The Trifecta: STEM, 3D Learning, and ART
22 December 2017 - Tricia Shelton
The Trifecta: STEM, 3D Learning, and ART

Equal opportunity, preparation for college and career, innovations to improve people’s lives, and a competitive US position in a global economy—- these are the needs that are driving a rethink of the approach to education.  Leading the transformation of education are initiatives like STEM and a vision for a new approach to teaching and learning […] [...]

A Changing Focus in Education
11 December 2017 - Tricia Shelton
A Changing Focus in Education

We are changemakers. We are learners. We are educators. A changing focus in education American education is in the midst of a major transformation, driven in large part, by a technology-focused global economy. Societal shifts like the Industrial Revolution had great impacts on education in the past. We can now ask ourselves, how is the […] [...]

Genetic Engineering & Biotechnology News Chooses BenchFly
9 December 2014 - Alan Marnett
Genetic Engineering & Biotechnology News Chooses BenchFly

We’re very excited to announce our new partnership with Genetic Engineering & Biotechnology News (GEN) to help bring two new video-based services to scientists. For more information, see the original release below. To learn more about how you can participate in Product Pioneers or Rising Stars of Science, please contact us at: info@benchfly.com. [...]

New Trend Alert: Using Video to Introduce Data
3 September 2014 - Emily Poulin
New Trend Alert: Using Video to Introduce Data

Here at BenchFly, we’re always looking for new ways to use video to improve scientific research. During a recent conference in Europe, the value of using video to simply introduce a research topic hit home with me. An exciting part of being a researcher of any level is the opportunity to travel to research conferences […] [...]

The ART of Video Funded by the Gates Foundation
22 August 2014 - Alan Marnett
The ART of Video Funded by the Gates Foundation

When we started BenchFly five years ago, in 2009, our mission was to make research a better career for current and future generations of scientists. Today we continue to work toward this goal using video as the primary means to educate scientists in companies, in universities and now in high schools! In the fall of 2013, we were incredibly […] [...]

copyright 2020 Bethany Huot/TheCOM,LLC / Powered by WordPress | theme SG Double
loading Cancel
Post was not sent - check your email addresses!
Email check failed, please try again
Sorry, your blog cannot share posts by email.